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专利名称：Reversible gelling culture media for in-vitro
cell culture in three- dimensional matrices
发明人：Yuehuei H. An,Vladimir A. Mironov,Anna
Gutowska
申请号：US09/062483申请日：19980417公开号：US06103528A公开日：20000815
摘要：A gelling cell culture medium useful for forming a three dimensional matrix forcell culture in vitro is prepared by copolymerizing an acrylamide derivative with ahydrophilic comonomer to form a reversible (preferably thermally reversible) gellinglinear random copolymer in the form of a plurality of linear chains having a plurality ofmolecular weights greater than or equal to a minimum gelling molecular weight cutoff,mixing the copolymer with an aqueous solvent to form a reversible gelling solution andadding a cell culture medium to the gelling solution to form the gelling cell culturemedium. Cells such as chondrocytes or hepatocytes are added to the culture medium toform a seeded culture medium, and temperature of the medium is raised to gel theseeded culture medium and form a three dimensional matrix containing the cells. Afterpropagating the cells in the matrix, the cells may be recovered by lowering thetemperature to dissolve the matrix and centrifuging.
申请人：BATTELLE MEMORIAL INSTITUTE,MEDICAL UNIVERSITY OF SOUTHCAROLINA
代理人：Paul W. Zimmerman
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